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May I ask, how it would be possible to show hiking paths, signs and so on at this theme?

Since GPXSee 13.47 (and the corresponding theme version) there are "Outdoor" and "Winter" layers in addition to the basic layer in the render theme.

And, can it be used for OAM and Locus Maps?

The theme has support for OAM-specific features. For using it in Locus, the symbol paths have to be adjusted and maybe a few other things that may cause minor rendering issues due to GPXSee and Locus using different render engines. But in general it is a standard, valid, Mapsforge render theme.

https://github.com/tumic0/GPXSee/blob/master/data/style/style.xml

The GPXSee Mapsforge/OpenAndroMaps render-theme uses a very similar cartographic style to Mapy.cz. The render-theme is a standard Mapsforge rendertheme so you should be able to use it in Locus but as GPXSee uses a different render engine than Mapsforge(Locus), you may have to adjust it a litle bit for Locus.